FAQs

Can you please advise if the 50 mL bioreactors from TPP can be used in the screening of micro carrier systems?

Do different flask sizes alter the mechanical stress of a culture?

Do you have any information on the optimal shaking diameter for the 50 mL bioreactor from TPP? We use a shaker with a choice of three shaking diameters: 12.5, 25, or 50 mm. I would like to know the best setting for volumes between 5 and 10 ml?

Why is the shaking diameter important?

Does the sterile plug influence the cultivation?

How can evaporation loss in a microtitre plate be prevented?

How can I determine if oxygen limitation occurs with my culture conditions?

How do my cultivation conditions influence oxygen transfer and evaporation rates in microplates?

I am not sure that the 50 mL Bioreactor from TPP will work for my application. How can I test it?

I am working with 24 square deepwell microplates. Do my cultivation conditions guarantee sufficient mixing and oxygen transfer?

I want to cultivate Escherichia coli in a microtitre plate. Which conditions should I choose?

Is a 15 ml bioreactor available? It could be quite useful for people who do a lot of small-scale stuff with volumes too big for 96 deep well plates but less than 10 ml.

Is it possible to cultivate animal cell cultures in a shaking flask?

Is it possible to grow adherent cells in the 50 mL Bioreactor from TPP?

Is it possible to incubate larger bioreactors than shake flasks on an orbital shaker?

Is the 50 mL bioreactor from TPP available with VENT caps?

Is there a difference between round and square wells?

Should the test tubes be fixed in a tilted position during the cultivation?

Should the tinfoil over the cotton plug be removed during the cultivation?

What can use to seal the openings of the 50 mL TPP-bioreactor?

What effect has the shaking diameter on the degree of mixing in a microplate?

What influence does sampling have on cultivations with a small filling volume?

What speed is recommended when working with the 50 mL bioreactor from TPP? At work volume of 15; 25; 35 ml?

Where can I find appropriate operating conditions for TubeSpin Bioreactors?

Which centrifuge or rotor accomodates the Bioreactor 600?

Which filling volume should be selected for the cultivation of E. coli in a 250 mL shake flask without baffles?

Which parameters determine the hydrodynamics inside the wells of an orbitally shaken microplate?