This is were you can find frequently asked questions about shakingtechnology. You can receive special information about different shaken bioreactors under the respective entry. If you cannot find a solution for your problem, please contact our "Dr. Shaker"-team of experts.

You should use 96-deepwell plates equipped with air-permeable sealing tape as a sterile barrier. The filling volume should not be greater than 500µL. Shake the plates with an orbital speed of 300 rpm and a shaking diameter of 50 mm. With smaller shaking diameters you should increase the shaking speed. For further information consider the following publication:
Characterization of Gas-Liquid Mass Transfer Phenomena in Microtiter Plates
Hermann R., Lehmann M., Buechs, J.
Biotechnology and Bioengineering (2003); Vol. 81, No. 2, pp 178- 186

On the one hand by using a shaking incubator with moisture control adjusted to 80-90 % humidity. On the other hand by choosing the appropriate sterile barrier. Additional data is available from the following publication:
Rapid Evaluation of Oxygen and Water Permeation through Microplate Sealing Tapes
H. F. Zimmermann, G. T. John, H. Trauthwein, U. Dingerdissen, K. Huthmacher
Biotechnol. Prog. (2003); Vol. 19, No. 3, pp 1061-1063

Generally, the oxygen transfer rate is better in a 96-square well microplate with the corners functioning as baffles [q.v. Hermann R., Lehmann M., Buechs, J., Biotech. Bioeng. (2003); Vol. 81, No. 2, pp 178-186].
Also available are new shaped wells (Flowerplate), which can further elevate the oxygen transfer. This kind of plate is only available in a 48-well format at the moment:
Microtiter plates as mini-bioreactors: miniaturization of fermentation methods
Duetz W A
Trends Microbiol. (2007); Vol.15, No. 10, pp 469-475